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ABSTRACT Peroxidase (EC 1.11.1.7) extracted from Gongronema latifolium was purified, on a two-step purification process of ammonium sulphate precipitation followed by dialysis. The enzyme was purified  6.8 fold with a specific activity of 2.04 when o-dianisidine was used as substrate. When the enzyme was subjected to different concentrations of hydrogen peroxide and o-dianisidine, the peak activity was 17.75µ/ml at  5mM for hydrogen peroxide and for o-dianisidine the peak activity was ...

ABSTRACT The study was conducted to evaluate the correlation of malaria parasite with ABO blood group and Rhesus factor in Nsukka and Enugu Metropolis. Three hundred blood samples were collected from Hansa Clinics, UNN Medical Center and UNTH, Enugu in which 169 were males and 131 were females between the ages of 1- 75 years. Full blood count was done using automated Homecue machine, Malaria parasite density was done using ...

ABSTRACT Methanol  seed  extract  of  Chrysophyllum  albidum  was  studied  to  ascertain  the  potential effects  of  the  extracts  on  some  biochemical   indices.  The  proximate   composition  of Chrysophyllum  albidum seed methanol extract was found to contain  crude protein (8.41± 0.02 mg/g), crude fat (19.12±0.01 mg/g), Ash content (1.42±0.01 mg/g) and moisture content (41.87± 0.03 mg/g). Results of the vitamin composition showed that it contains vitamin C (99.0± 0.03 µ/100g) vitamin A (70.0±0.01 ...

ABSTRACT The aim of this study was to convert fresh cassava wastewater into bio-fertilizer in a liquid phase anaerobic bio-reactor. Fresh cassava wastewater was collected from a garri mill company at Ogurugu road, in Nsukka Local Government Area of Enugu State, Nigeria. The fresh cassava wastewater was sieved using a mesh (0.154 mm aperture) to remove large solid particles and debris and the filtrate stored at 4◦C. Filtered fresh cassava ...

ABSTRACT Musa paradisiacae  commonly known as plantain is a rhizomatous  perennial  crop used as a source of starchy staple for millions of people in Nigeria. Different parts ofthe plant have been used in the treatment of various ailments and there are claims that it has antidiarrhoeal activity. This study is therefore aimed at determining the effects of  unripe  Musa  paradisiacae  pulp  and  peel  homogenates  on  castor  oil-induced diarrhoea in Wistar ...

ABSTRACT The clinical resistance of Candida species to antifungal medications, particularly fluconazole, is rising. A major mechanism responsible for this resistance is the alteration in the nucleotide sequence of the gene that codes for the ERG11 protein, a key enzyme in the ergosterol synthesis pathway, which remains the target of fluconazole. This study investigated the distribution of different species of Candida in human and dog vaginal swabs, the susceptibility of ...

ABSTRACT Pregnancy induced hypertensive disorders have become very common medical complication in Nigeria  with  its  attendant  morbidity  and  mortality.  The  morbidity  and  mortality  may  be associated with its possible effect on the kidneys which was studied. Results of the electrolyte changes from this study showed that there was non-significant difference (p>0.05) in sodium ion concentration of subjects with pregnancy induced hypertension in second and third trimesters (groups 4 and 5 ...

ABSTRACT Millettia aboensis leaf is a rich source of reducing sugar, tannins, glycosides and flavonoids and has been reported to have medicinal property as well as some physiological potentials. The leaf also has been used by traditional herbalists for general healing of diseases  including ulcer and laxatives. This study evaluated the effect of aqueous extract of M.   aboensis leaves on lomotil- induced  constipation  in Wistar  albino rats. The qualitative  phytochemical ...

ABSTRACT Peroxidase activity from G. latifolium was done to see whether it could be used in industries. Crude peroxidase was extracted from G. latifolium with 0.05M sodium phosphate buffer of pH 6.0; 70% ammonium sulphate  saturation to  precipitated  protein with  the  highest  G.  latifolium  peroxidase activity.  After  gel  filtration,  two  major  peaks  were  seen  and  the  active  fractions  were  pooled differently together and characterized. The optimal pH for the enzyme ...

ABSTRACT The efficacy of Uvaria chamae plant species in herbal remedies may have come as a result of trial  and  error.  This  could  be  as  a  result  of  poor  information  on  the  phytochemistry, antioxidant and toxicity of this plant parts. The present study compares the in vitro and in vivo antioxidant potentials and toxicities of methanol extracts of Uvaria chamae leaves and roots. Results of in vitro antioxidant potentials revealed ...

ABSTRACT Alcohol  has many biological actions,  and adverse  effects on lipid metabolism.  The  main objective of this present study was designed to study the effects of alcohol administration on serum   lipid profile, total protein, liver enzymes and histopathological effect in rats. A total twenty five male wistar rats (190-200g) were divided into five groups of five animals each. The animals were grouped so that the mean difference in the various ...

ABSTRACT Crude haemoglobins were extracted from blood samples of identified individuals of normal (AA), sickle trait carrier (AS), and sickle (SS) by employing centrifugation techniques. The crude haemoglobins were dialysed at 4oC for 12hr against 50mM Tris-HCl buffer of pH 7.2. The effects of pyrimethamine and sulphadoxine on the haemoglobins in the presence and absence of sodium dodecyl sulphate (SDS) were studied at pH 5.0 and 7.2 with uv-visible titration ...

ABSTRACT Data on the current levels of sodium consumption of Nigerian  adults are  urgently needed  to  determine  the  contribution  of  dietary  sodium  as  a  risk  factor  in  the increasing incidence of hypertension and related complications  in the country. The aim of this study was  to  estimate  the daily salt  intake of healthy ambulant Nigerian adults  using  the  24-hour  urinary  sodium ion excretion method (the gold standard) and  spot urine  sodium ...

ABSTRACT This study is therefore set to evaluate the effect of the effluent discharged from the ceiling industry into the immediate ecosystem; .Ekwuru river. The physicochemical characteristics of the effluent, at the discharge points at Ekwuru river were analyzed. Two suspected groups of pollutants of concern namely Polycyclic aromatic hydrocarbons (PAHs) and Heavy metals were investigated in the samples using Gas chromatograpy and Atomic absorption spectrophotometer. A reference site 20km ...

ABSTRACT Sickle cell disease (SCD) is a major problem of the developing world. The search for anti- sickling agent is of particular interest since plant bioactive agents are said to be of medicinal significance. This research investigated aqueous extract of Sphenostylis stenocarpa seed for possible in-vitro anti-sickling properties. Induction of sickling and polymerization of HbSS was done by addition of 3.4ml of metabisulphite (Na2S2O2) solution. Further analyzes carried out on ...

ABSTRACT Beta-amylase was extracted from fresh sweet potato (Ipomoea batatas) stem and was partially purified  using  60%  ammonium  sulphate  saturation  and  gel  filtration  chromatography.  The specific activity for the crude enzyme was found to be 95.56±2.8 U/mg with the purification fold of 1, while the protein concentration was 0.5 mg/ml. After gel filtration chromatography, the specific activity obtained was 186.37±2.56 U/mg with purification fold of 2 at 13.3% recovery. The ...

ABSTRACT Polythene and PVC are polymers used in production of packaging materials which are frequently used for food and water. Antioxidants, stabilizers, plasticizers, lubricants, antimicrobials, anti- static and anti-blocking agents, “slips,” or heat resistance agents are usually incorporated during the production of these packaging materials to improve functionality. A wide variety of synthetic and naturally occurring polymers absorb solar ultraviolet radiation and undergo photolytic, photooxidative, and thermooxidative reactions that result ...

ABSTRACT The aim of this study was to isolate, partially purify and characterize α-Amylase from Bacillus alcalophilus. The enzyme (α-amylase) was isolated from Bacillus alcalophilus using cassava as only carbon source. 20 g of soil sample were weighed out and dissolved in 40 ml of distilled water in a clean conical flask, mixed vigorously and heated at 60oC for 60 min in water bath and taken as the stock culture. ...

ABSTRACT Medicinal plants have been assumed to be good in detoxifications because they contain bioactive compounds capable of doing this. However, some plants are very toxic to both humans and animals with the potential of damaging certain organs in the body. Many plant- derived  substances,  collectively termed  “phytochemicals,”  are  becoming  increasingly known for their antioxidant activity. This study is aimed at investigating the toxic and as well as the antioxidant ...

ABSTRACT This study investigated the effect of cocoa polyphenol on isoproterenol-induced myocardial infarction in Wistar rats. Polyphenol was extracted from cocoa using a hydroalcohol solvent. Forty albino rats divided into six groups of four rats each were used for the study. Groups 3, 4, 5 and 6 were pretreated with the extract (300, 500 and 700 mg/kg) and 100 mg/kg of atenolol (standard drug) respectively before administration with isoproterenol. Group ...