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EFFECT OF SUCROSE CONCENTRATIONS ON THE IN VITRO PLANTLET REGENERATION FROM MATURE ZYGOTIC EMBRYO EXPLANTS OF JATROPHA CURCAS L. IN MURASHIGE AND SKOOG (MS) MEDIUM

ABSTRACT Jatropha curcas L. (physic nut) is a multipurpose plant which belongs to the  family  Euphorbiaceae.  The  non-edible  oil  produced  from  the  seeds  is considered a potential renewable source of biodiesel. This research aims at estimating the optimum sucrose concentration for in vitro plantlet regeneration using mature zygotic embryos as explants in Murashige and Skoog (MS) media. Sucrose was employed as carbon source at concentrations ranging from 0 to 5 ...

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EFFECTS OF SIMULATED CRUDE OIL CONTAMINATION ON THE PHYSICOCHEMICAL PROPERTIES AND BACTERIAL POPULATION OF RHIZOSPHERE OF SORGHUM VULGARE PERS.

ABSTRACT This study has been undertaken to investigate the physicochemical properties and the bacterial population of the rhizosphere of Sorghum vulgare. In order to simulate spillage, 0.2, 0.9 and 5.0 % v/w concentrations of crude oil were used to contaminate soil sown with seeds of Sorghum vulgare, while the control had no crude oil contamination.  0ne hundred and twenty days after contamination, the physicochemical properties and bacterial population of the ...

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ASPECTS OF HOLOCENE VEGETATION RECONSTRUCTION OF SOUTH EASTERN NIGERIA POLLEN RECORD FROM OZZI LAKE OPI NSUKKA ENUGU STATE

ABSRACT A palynological study of the Palaeo-vegetation of Opi Nsukka, north eastern Enugu State was investigation through a palyno-analysis of 1.5 m lake sediment core collected from Ozzi Lake, Ugwu Ozioko Opi- Agu Nsukka Enugu State. The pollen flora showed that a total of 52 pollen types belonging to 30 families were recorded. Fungal and fern spores in addition to charred plant particles were also recorded from the sediment profile. ...

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GROWTH DEVELOPMENT AND HEAVY METAL UPTAKE BY AMARANTHUS HYBRIDUS L. POLLUTED WITH PHARMACEUTICAL EFFLUENTS

ABSTRACT Experiments were carried out to study the ability of Amaranthus hybridus to accumulate heavy metals and active pharmaceutical  ingredients  (APis) from two pharmaceutical  effluents samples (effluent P, containing Paracetamol and effluent Bcontaining Amoxicillin). The preliminary experiment was carried out in a screen house in completely  randomized  design (CRD), using sterilised  soil and controlled irrigation with the effluents while distilled water was the control. The main experiment involved natural attenuation ...

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MORPHO-ANATOMICAL STUDY OF TWO CLOSELY RELATED SPECIES OF OLDENLANDIA (RUBIACEAE) IN NIGERIA

ABSTRACT Morpho-anatomical study of Oldenlandia  corymbosa  L.  and  Oldenlandia  affinis (Roem & Schult.)  DC.  in  the  family  Rubiaceae  was  undertaken  in  order  to  assess  the  taxonomic relationship of the two species. Ten samples of each of the two species were randomly collected from their natural regions of provenance, Nkalagu (derived Savanna), Nsukka (derived Savanna), and Otukpo (Southern Guinea Savanna). Both species are herbaceous and are therefore, apomorphous than shrubs and ...

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HYDROCARBON DEGRADATION AND ACCUMULATION BY SANSEVIERIALIBERICA GEROME ANDLABROY POLLUTED WITH CRUDE OIL

ABSTRACT The  capability  ofSansevierialiberica   to  withstand  crude  oil  pollution,  degrade   and/or accumulate the contaminant was investigated using 0.3, 1.3 and 6.3% v/w concentrations of crude oil to pollute soil vegetated with the stem cuttings of the plant. These treatments were repeated in unvegetated soils and the control had no crude oil pollution. The experiment was carried out in 3 replicates in a completely randomized design. Total Petroleum Hydrocarbons (TPH) were ...

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MULTI-LOCATION ENVIRONMENTAL STUDIES OF YIELD AND ANTIOXIDANT PROPERTIES OF SEVEN VARIETIES OF SWEET POTATO (IPOMOEA BATATAS (L) LAM)

ABSTRACT The  chronic problem of fluctuations  in environmental conditions coupled with the  lack  of cultivar evaluations over a wide range of environments, have limited the rapid development of elite sweet potato (Ipomoea batatas (L) Lam.) cultivars across different agro-ecological locations of Nigeria. To evaluate the multi-locational environmental performance of sweet potato over three agro-ecolological locations, (Mbano, Nsukka and Umudike) in Eastern Nigeria, seven local elite  sweet  potato  varieties Ex-Onyunga, Centina,  ...

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EFFECTS OF CRUDE OIL ON SOME BACTERIA AND MINERAL CONSTITUENTS OF RHIZOSPHERE OF DELONIX REGIA HOOK

ABSTRACT Crude oil spill is hazardous to soil and subsequently affects food production. Certain plants however tolerate and/or improve the mineral deficiency imposed by crude oil. Also, due to the ability of bacteria to degrade crude oil, plants that enhance the abundance of soil bacteria under pollution have been under investigation. The effects of crude oil on the bacterial count and mineral constituents of soil rhizosphere of  Delonixregia  were  investigated. ...

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DETERMINATION OF ELECTROLYTES AND GLYCOSYLATED HAEMOGLOBIN CONCENTRATIONS IN DIABETES MELLITUS PATIENTS IN GWAGWALADA AREA COUNCIL ABUJA

ABSTRACT Diabetes is a major threat to global public health, and the numbers of diabetic patients are rapidly increasing world-wide.  This study was aimed at determining the serum  electrolyte concentration and glycosylated haemoglobin level of diabetic patients and apparently healthy individuals.  A total of one hundred  and  twenty  (120)  subjects  were  used  for  the  study. Seventy (70) subjects (apparently healthy) aged between 38 and 60 years with no history of ...

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DECOLORIZATION OF SYNTHETIC DYE USING PARTIALLY PURIFIED PEROXIDASE FROM GREEN CABBAGE (BRASSICA OLERACEA)

ABSTRACT Peroxidase  was  extracted  from  cabbage  and  was  purified  in  three  different purification processes. It was first purified by ammonium sulphate precipitation and highest peroxidase activity was observed at 80% saturation.  Hence, 80% saturation was used to mass produce the enzyme. The enzyme was  again purified by dialysis which tends to remove salt as impurity from the  precipitated  enzyme. The enzyme was further purified by gel filtration which further removed ...

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IMPACT OF FRESH AND FERMENTED PALM OIL MILL EFFLUENTS ON SOIL PHYSICOCHEMICAL PARAMETERS AND ENZYME ACTIVITIES IN UMUAKA NJABA IMO STATE OF NIGERIA

ABSTRACT The impact of palm oil mill effluents (POME) on soil fertility was determined by studying the physico-chemical and heavy metals parameters found in fresh and fermented POME from six palm oil milling sites. Some soil enzymes (like catalase, dehydrogenase and lipase) and physico-chemical parameters of POME polluted soil wereevaluated from six dumpsites and soil from ten-yards distance from dumpsites in relation to the control farmland in the area. The ...

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EFFECT OF WATER ACTIVITY ON TRANSESTERIFICATION KINETIC PARAMETERS OF COCONUT OIL WITH ETHANOL

ABSTRACT This work is focused on determining quantitatively how water, even in its smallest amount, affects  transesterification kinetic  parameters  of  coconut  oil  with  ethanl;  with  the  view  of assessing whether it is preferable to use anhydrous ethanol with high price or ethanol containing a certain amount of water in biodiesel production process. Physicochemical properties of the coconut oil were also determined. The coconut oil produced was yellow and had the ...

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EFFECTS OF CHEMICALS USED IN STORING MAIZE ON IT S ZEIN CONTENT OVER A DURATION OF TIME

Abstract Maize is the largest and most important agricultural commodity in the world.  Zein is the storage protein in maize known as prolamine.  It is water insoluble  and forms odorless,  tasteless,  clear edible films.  The zein content of maize is 25% of the dry weight of maize seeds, it is resistant to hydrolysis by  very  dilute acids and  contains the  following amino  acids:  glutamic acid  and glutamine,  leucine,  proline,  alanine, ...

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EFFECT OF FRACTION I OF ABRUSPRECATORIUS SEED METHANOL EXTRACT ON PARACETAMOL-INDUCED LIVER DAMAGE IN RATS

ABSTRACT The aim of this study was to investigate the prophylactic and curative effects of an alkaloid-rich fraction of Abrusprecatorius seedchloroform-methanol extract on paracetamol-induced hepatotoxicity in rats. The percentage yield of the methanol extract of Abrusprecatorius seeds was found to be 2.08% w/w. Further purification of the extract using Sephadex gel G15 to get a purer  sample was done.  The  fractions were  spotted on a  TLC plate  and  was spread ...

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EFFECT OF THE ETHANOL EXTRACT OF ZAPOTECA PORTORICENSIS ROOTS ON MALARIA-INFECTED MICE

ABSTRACT The effect of the Zapoteca portoricensis roots on malaria was determined by checking the percentage parasitaemia, some haematological parameters such as packed cell volume (PCV), haemoglobin concentration (Hb), total white blood cell count (TWBC) and red blood cell count (RBC), also some biochemical parameters such as liver enzyme markers; aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) and kidney function markers which include urea and creatinine concentrations. ...

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STUDIES ON THE ANTIDIARRHOEAL AND ANTIMICROBIAL EFFECT OF THE METHANOL EXTRACT OF HARUNGANA MADAGASCARIENSIS LEAVES

ABSTRACT Harugana.madagascariensis  leaf  is  a  rich  source  of  flavonoid  saponin,carbohydratesterpenoid, steroids, phenolics,tannin and   has been reported to have medicinal property as well as some physiological potentials. This study evaluated the antidiarrhoeal and antimicrobial activity of methanol extract of Harugana madagascariensis leaves. The qualitative phytochemical constituents of the extract showed the relative presence of flavonoids   in relatively high concentration, tannins,     reducing sugars, steroids, phenols, alkanoids, terpenoids and saponins in moderately high ...

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PARTIAL PURIFICATION AND CHARACTERIZATION OF AMYLASE FROM GERMINATED AFRICAN YAM BEAN SEEDS (SPHENOSTYLIS STENOCARPA)

ABSTRACT The activity of α-amylase and protein concentration in African yam bean seeds increases as germination progresses up to day 8 of germination where it exhibited its highest level, followed by sequenced decreased activity and protein concentration till day 12. Starch from African yam bean seeds, corn and cassava were used for the hydrolysis experiment. There was a significant activity of α-amylase in each of the substrate used, however, the ...

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COMPARATIVE PROPERTIES OF THE METHYL AND ETHYL ESTERS PRODUCED FROM AVOCADO (PERSEA AMERICANA) PULP OIL

ABSTRACT Biodiesel  was  produced  from  Avocado  (Persea  Americana)  pulp  oil  by  trans esterification  with  methanol  and  ethanol  (6:1  methanol/ethanol-to-oil  ratio)  using 0.09% sodium hydroxide as catalyst in 60 minutes reaction time. The percentage yield of the methyl ester was 78% while that of the ethyl ester yield was 66%. Avocado pulp   oil  (APO)   biodiesel   obtained   in   each   case   showed   good   qualities   on characterization with the fuel properties showing good qualities ...

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PREVALENCE AND SEVERITY OF HYPOGLYCAEMIA AND LACTIC ACIDOSIS IN CHILDREN DIAGNOSED WITH PLASMODIUM FALCIPARUM MALARIA

ABSTRACT The prevalence and severity of hypoglycaemia and lactic acidosis in Nigerian children diagnosed with Plasmodium falciparum malaria were determined in 100 outpatient children aged 3-144 months (12 years). The children were grouped into 2 categories: 3-59 month old and 60-144 month old. The results obtained indicated that out of the 100 children recruited into this study, seventy-five (75%) were infected while twenty-five (25%) were uninfected with Plasmodium falciparum malaria. ...

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LIPID PROFILE OF PYTHON FAT AND ITS ANTI-INFLAMMATORY POTENTIALS

ABSTRACT Ethnozoological studies have shown that Python sebae, is associated with medicinal uses in Nigeria. This study was designed to determine the chemical composition of the oil extracted from the body fat of Python sebae and to evaluate its anti-inflammatory potentials. The Python fat used in the study were obtained from two (2) locations and were tagged: Fat Python A (FPA) and Fat Python D (FPD). The fatty acid profile ...

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