Download Category: BIO-CHEMISTRY PROJECT TOPICS

ABSTRACT Peroxidase activity from G. latifolium was done to see whether it could be used in industries. Crude peroxidase was extracted from G. latifolium with 0.05M sodium phosphate buffer of pH 6.0; 70% ammonium sulphate  saturation to  precipitated  protein with  the  highest  G.  latifolium  peroxidase activity.  After  gel  filtration,  two  major  peaks  were  seen  and  the  active  fractions  were  pooled differently together and characterized. The optimal pH for the enzyme ...

ABSTRACT The efficacy of Uvaria chamae plant species in herbal remedies may have come as a result of trial  and  error.  This  could  be  as  a  result  of  poor  information  on  the  phytochemistry, antioxidant and toxicity of this plant parts. The present study compares the in vitro and in vivo antioxidant potentials and toxicities of methanol extracts of Uvaria chamae leaves and roots. Results of in vitro antioxidant potentials revealed ...

ABSTRACT Alcohol  has many biological actions,  and adverse  effects on lipid metabolism.  The  main objective of this present study was designed to study the effects of alcohol administration on serum   lipid profile, total protein, liver enzymes and histopathological effect in rats. A total twenty five male wistar rats (190-200g) were divided into five groups of five animals each. The animals were grouped so that the mean difference in the various ...

ABSTRACT Crude haemoglobins were extracted from blood samples of identified individuals of normal (AA), sickle trait carrier (AS), and sickle (SS) by employing centrifugation techniques. The crude haemoglobins were dialysed at 4oC for 12hr against 50mM Tris-HCl buffer of pH 7.2. The effects of pyrimethamine and sulphadoxine on the haemoglobins in the presence and absence of sodium dodecyl sulphate (SDS) were studied at pH 5.0 and 7.2 with uv-visible titration ...

ABSTRACT Data on the current levels of sodium consumption of Nigerian  adults are  urgently needed  to  determine  the  contribution  of  dietary  sodium  as  a  risk  factor  in  the increasing incidence of hypertension and related complications  in the country. The aim of this study was  to  estimate  the daily salt  intake of healthy ambulant Nigerian adults  using  the  24-hour  urinary  sodium ion excretion method (the gold standard) and  spot urine  sodium ...

ABSTRACT This study is therefore set to evaluate the effect of the effluent discharged from the ceiling industry into the immediate ecosystem; .Ekwuru river. The physicochemical characteristics of the effluent, at the discharge points at Ekwuru river were analyzed. Two suspected groups of pollutants of concern namely Polycyclic aromatic hydrocarbons (PAHs) and Heavy metals were investigated in the samples using Gas chromatograpy and Atomic absorption spectrophotometer. A reference site 20km ...

ABSTRACT Sickle cell disease (SCD) is a major problem of the developing world. The search for anti- sickling agent is of particular interest since plant bioactive agents are said to be of medicinal significance. This research investigated aqueous extract of Sphenostylis stenocarpa seed for possible in-vitro anti-sickling properties. Induction of sickling and polymerization of HbSS was done by addition of 3.4ml of metabisulphite (Na2S2O2) solution. Further analyzes carried out on ...

ABSTRACT Beta-amylase was extracted from fresh sweet potato (Ipomoea batatas) stem and was partially purified  using  60%  ammonium  sulphate  saturation  and  gel  filtration  chromatography.  The specific activity for the crude enzyme was found to be 95.56±2.8 U/mg with the purification fold of 1, while the protein concentration was 0.5 mg/ml. After gel filtration chromatography, the specific activity obtained was 186.37±2.56 U/mg with purification fold of 2 at 13.3% recovery. The ...

ABSTRACT Polythene and PVC are polymers used in production of packaging materials which are frequently used for food and water. Antioxidants, stabilizers, plasticizers, lubricants, antimicrobials, anti- static and anti-blocking agents, “slips,” or heat resistance agents are usually incorporated during the production of these packaging materials to improve functionality. A wide variety of synthetic and naturally occurring polymers absorb solar ultraviolet radiation and undergo photolytic, photooxidative, and thermooxidative reactions that result ...

ABSTRACT The aim of this study was to isolate, partially purify and characterize α-Amylase from Bacillus alcalophilus. The enzyme (α-amylase) was isolated from Bacillus alcalophilus using cassava as only carbon source. 20 g of soil sample were weighed out and dissolved in 40 ml of distilled water in a clean conical flask, mixed vigorously and heated at 60oC for 60 min in water bath and taken as the stock culture. ...

ABSTRACT Medicinal plants have been assumed to be good in detoxifications because they contain bioactive compounds capable of doing this. However, some plants are very toxic to both humans and animals with the potential of damaging certain organs in the body. Many plant- derived  substances,  collectively termed  “phytochemicals,”  are  becoming  increasingly known for their antioxidant activity. This study is aimed at investigating the toxic and as well as the antioxidant ...

ABSTRACT This study investigated the effect of cocoa polyphenol on isoproterenol-induced myocardial infarction in Wistar rats. Polyphenol was extracted from cocoa using a hydroalcohol solvent. Forty albino rats divided into six groups of four rats each were used for the study. Groups 3, 4, 5 and 6 were pretreated with the extract (300, 500 and 700 mg/kg) and 100 mg/kg of atenolol (standard drug) respectively before administration with isoproterenol. Group ...

ABSTRACT Hepatitis B caused by the Hepatitis B virus (HBV) is one of the most common infectious diseases in the world and a major health problem. Its preventive measure is seen in hepatitis B vaccine. The inability of alum adjuvanted hepatitis B vaccine to trigger Th1 response and the need for reduced booster doses necessitate the quest for novel adjuvant. The present study is  aimed  at  evaluating  the  immunogenicity  of  ...

ABSTRACT This study was aimed at evaluating oxidative stress and mitochondrial dysfunction in HIV patients on a combination of emtricitabine, tenofovir and efavirenz (ATRIPLA), commonly used for treating these patients attending the HIV clinic of Enugu State University Teaching (ESUT) Hospital, Parklane, Enugu, Nigeria following short and long-term therapy. Ninety six (96) subjects (divided into four groups) aged between 18 and 60 years were recruited for the study from the ...

ABSTRACT The effect of  alkaline steep and air-rest cycle on the development of  peroxidase activity during malting  was  investigated  in  sorghum  variety,  KSV8.  Preliminary  experiment  showed  that alkaline steep (test) and the distilled water steep (control) had germinative energy of 92± 2.87 % and 89± 0.57 % respectively. In regime II (sorghum grains steeped in distilled water for 24h), both the test experiment and the control had germinative energy of ...

ABSTRACT This study was conducted  to measure the glycemic  index and blood glucose response  of staple foods consumed in Nigeria in healthy Wistar rats. The test food samples Maize (Zea mays),  Wheat  (Triticum  spp),  Acha  (Digitaria  exilis)  and  Oat  (Elkris  super  oat)  were formulated with palm kernel cake, rice husk/bran, soya beans, crayfish, bone meal, premix and  salt  into  diets in the  laboratory of the Department  of  Home  Science,  Nutrition ...

ABSTRACT Naringin was extracted from lemon peels with extraction yield of 0.25%. Two assay methods were used to assay for naringinase activity, one is naringin disappearance assay method and the other is reducing sugars appearance assay method. The naringinase activities for the crude enzyme were found to be 157.7 µmol/min using naringin disappearance method and 493.64 µmol/min using reducing sugars appearance method. Ammonium sulphate saturation (50%) was found suitable for ...

ABSTRACT The use of medicinal plants for the treatment of many diseases is associated with folk medicine from different parts of the World. However, information on the toxicology of these plants part used in Nigeria folk medicine is rare. Thus, this work is aimed at revealing a range of phytochemicals in the Phyllanthus amarus plant, the antioxidant constituents and its toxicologic effects on some important biochemical parameters in male albino ...

ABSTRACT Natural products are sources of bioactive compounds and have potential for developing some novel therapeutic agent, due to their enormous chemical diversity. These diverse natural products form the basis of many traditional medicine systems throughout the world, due to their accessibility,   efficacy   and   minimal   side   effects.   The   present   study   evaluated   the antiinflammatory, antipyretic and analgesic properties of Vitex simplicifolia leaf. The extraction of 464.67 g of Vitex simplicifolia leaves ...

ABSTRACT Glucoamylases  were  produced  from both  plants  and microorganisms  and were  optimized  for starch hydrolysis in batch bioreactor. Amylase activity was monitored  in germinating guinea com seeds for seven days.  Highest  amylase  activity  was observed  on days  3  and 7.  A study of the amylopectin content of millet, guinea com, cassava, com and tigernut starch showed that tiger nut had the  highest  amylopectin  content  while  cassava  starch had the  lowest. ...