Download Category: BIO-CHEMISTRY PROJECT TOPICS


DETERMINATION OF ELECTROLYTES AND GLYCOSYLATED HAEMOGLOBIN CONCENTRATIONS IN DIABETES MELLITUS PATIENTS IN GWAGWALADA AREA COUNCIL ABUJA

ABSTRACT Diabetes is a major threat to global public health, and the numbers of diabetic patients are rapidly increasing world-wide.  This study was aimed at determining the serum  electrolyte concentration and glycosylated haemoglobin level of diabetic patients and apparently healthy individuals.  A total of one hundred  and  twenty  (120)  subjects  were  used  for  the  study. Seventy (70) subjects (apparently healthy) aged between 38 and 60 years with no history of ...

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DECOLORIZATION OF SYNTHETIC DYE USING PARTIALLY PURIFIED PEROXIDASE FROM GREEN CABBAGE (BRASSICA OLERACEA)

ABSTRACT Peroxidase  was  extracted  from  cabbage  and  was  purified  in  three  different purification processes. It was first purified by ammonium sulphate precipitation and highest peroxidase activity was observed at 80% saturation.  Hence, 80% saturation was used to mass produce the enzyme. The enzyme was  again purified by dialysis which tends to remove salt as impurity from the  precipitated  enzyme. The enzyme was further purified by gel filtration which further removed ...

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IMPACT OF FRESH AND FERMENTED PALM OIL MILL EFFLUENTS ON SOIL PHYSICOCHEMICAL PARAMETERS AND ENZYME ACTIVITIES IN UMUAKA NJABA IMO STATE OF NIGERIA

ABSTRACT The impact of palm oil mill effluents (POME) on soil fertility was determined by studying the physico-chemical and heavy metals parameters found in fresh and fermented POME from six palm oil milling sites. Some soil enzymes (like catalase, dehydrogenase and lipase) and physico-chemical parameters of POME polluted soil wereevaluated from six dumpsites and soil from ten-yards distance from dumpsites in relation to the control farmland in the area. The ...

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EFFECT OF WATER ACTIVITY ON TRANSESTERIFICATION KINETIC PARAMETERS OF COCONUT OIL WITH ETHANOL

ABSTRACT This work is focused on determining quantitatively how water, even in its smallest amount, affects  transesterification kinetic  parameters  of  coconut  oil  with  ethanl;  with  the  view  of assessing whether it is preferable to use anhydrous ethanol with high price or ethanol containing a certain amount of water in biodiesel production process. Physicochemical properties of the coconut oil were also determined. The coconut oil produced was yellow and had the ...

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EFFECTS OF CHEMICALS USED IN STORING MAIZE ON IT S ZEIN CONTENT OVER A DURATION OF TIME

Abstract Maize is the largest and most important agricultural commodity in the world.  Zein is the storage protein in maize known as prolamine.  It is water insoluble  and forms odorless,  tasteless,  clear edible films.  The zein content of maize is 25% of the dry weight of maize seeds, it is resistant to hydrolysis by  very  dilute acids and  contains the  following amino  acids:  glutamic acid  and glutamine,  leucine,  proline,  alanine, ...

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EFFECT OF FRACTION I OF ABRUSPRECATORIUS SEED METHANOL EXTRACT ON PARACETAMOL-INDUCED LIVER DAMAGE IN RATS

ABSTRACT The aim of this study was to investigate the prophylactic and curative effects of an alkaloid-rich fraction of Abrusprecatorius seedchloroform-methanol extract on paracetamol-induced hepatotoxicity in rats. The percentage yield of the methanol extract of Abrusprecatorius seeds was found to be 2.08% w/w. Further purification of the extract using Sephadex gel G15 to get a purer  sample was done.  The  fractions were  spotted on a  TLC plate  and  was spread ...

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EFFECT OF THE ETHANOL EXTRACT OF ZAPOTECA PORTORICENSIS ROOTS ON MALARIA-INFECTED MICE

ABSTRACT The effect of the Zapoteca portoricensis roots on malaria was determined by checking the percentage parasitaemia, some haematological parameters such as packed cell volume (PCV), haemoglobin concentration (Hb), total white blood cell count (TWBC) and red blood cell count (RBC), also some biochemical parameters such as liver enzyme markers; aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) and kidney function markers which include urea and creatinine concentrations. ...

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STUDIES ON THE ANTIDIARRHOEAL AND ANTIMICROBIAL EFFECT OF THE METHANOL EXTRACT OF HARUNGANA MADAGASCARIENSIS LEAVES

ABSTRACT Harugana.madagascariensis  leaf  is  a  rich  source  of  flavonoid  saponin,carbohydratesterpenoid, steroids, phenolics,tannin and   has been reported to have medicinal property as well as some physiological potentials. This study evaluated the antidiarrhoeal and antimicrobial activity of methanol extract of Harugana madagascariensis leaves. The qualitative phytochemical constituents of the extract showed the relative presence of flavonoids   in relatively high concentration, tannins,     reducing sugars, steroids, phenols, alkanoids, terpenoids and saponins in moderately high ...

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PARTIAL PURIFICATION AND CHARACTERIZATION OF AMYLASE FROM GERMINATED AFRICAN YAM BEAN SEEDS (SPHENOSTYLIS STENOCARPA)

ABSTRACT The activity of őĪ-amylase and protein concentration in African yam bean seeds increases as germination progresses up to day 8 of germination where it exhibited its highest level, followed by sequenced decreased activity and protein concentration till day 12. Starch from African yam bean seeds, corn and cassava were used for the hydrolysis experiment. There was a significant activity of őĪ-amylase in each of the substrate used, however, the ...

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COMPARATIVE PROPERTIES OF THE METHYL AND ETHYL ESTERS PRODUCED FROM AVOCADO (PERSEA AMERICANA) PULP OIL

ABSTRACT Biodiesel  was  produced  from  Avocado  (Persea  Americana)  pulp  oil  by  trans esterification  with  methanol  and  ethanol  (6:1  methanol/ethanol-to-oil  ratio)  using 0.09% sodium hydroxide as catalyst in 60 minutes reaction time. The percentage yield of the methyl ester was 78% while that of the ethyl ester yield was 66%. Avocado pulp   oil  (APO)   biodiesel   obtained   in   each   case   showed   good   qualities   on characterization with the fuel properties showing good qualities ...

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PREVALENCE AND SEVERITY OF HYPOGLYCAEMIA AND LACTIC ACIDOSIS IN CHILDREN DIAGNOSED WITH PLASMODIUM FALCIPARUM MALARIA

ABSTRACT The prevalence and severity of hypoglycaemia and lactic acidosis in Nigerian children diagnosed with Plasmodium falciparum malaria were determined in 100 outpatient children aged 3-144 months (12 years). The children were grouped into 2 categories: 3-59 month old and 60-144 month old. The results obtained indicated that out of the 100 children recruited into this study, seventy-five (75%) were infected while twenty-five (25%) were uninfected with Plasmodium falciparum malaria. ...

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LIPID PROFILE OF PYTHON FAT AND ITS ANTI-INFLAMMATORY POTENTIALS

ABSTRACT Ethnozoological studies have shown that Python sebae, is associated with medicinal uses in Nigeria. This study was designed to determine the chemical composition of the oil extracted from the body fat of Python sebae and to evaluate its anti-inflammatory potentials. The Python fat used in the study were obtained from two (2) locations and were tagged: Fat Python A (FPA) and Fat Python D (FPD). The fatty acid profile ...

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PRODUCTION AND CHARACTERIZATION OF BIODIESEL FROM LUFFA CYLINDRICA SEED OIL

ABSTRACT Biodiesel  was  produced  from  the  seed  oil  of  Luffa  cylindrica.  The  oil  obtained  was transesterified to produce methyl-esters and glycerol. The percentage oil yield of 36.32% was obtained from Luffa cylindrica seed. Biodiesel properties of methyl-esters were determined using American Society for Testing and Materials (ASTM) Standards and compared with that of petrodiesel. The methyl-ester yield of 92.06 % was obtained from Luffa cylindrica seedoil. Higher viscosity at 40oC ...

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ISOLATION PARTIAL PURIFICATION AND CHARACTERIZATION OF GLUCOAMYLASE FROM ASPERGILLUS NIGER IN SUBMERGED FERMENTATION USING AMYLOPECTIN FROM TIGER NUT STARCH AS CARBON SOURCE

ABSTRACT Glucoamylase  was  isolated  from  Aspergillus  niger  and  purified  using  ammonium sulphate precipitation and  gel  filtration respectively.  The  purified  enzyme was then characterized to determine the optimum conditions required by the produced enzyme. A fourteen day pilot study was carried out to determine the day of isolation of crude with highest glucoamylase activity. Day 5 and 12 had highest glucoamylase activity. The specific activities for the crude enzyme were found ...

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STUDIES ON THE PHYTOCHEMICAL AND NUTRITIONAL COMPOSITION OF AQUEOUS LEAF EXTRACT OF JUSTICIA CARNEA AND ITS EFFECT ON SOME BIOCHEMICAL PARAMETERS IN ANAEMIC RATS.

ABSTRACT Anaemia is a common blood disorder that affects people of all ages, though people at greater risk are the elderly, young women and infants. Different drugs are used but many depend mainly on plants and herbal products for its treatment. In this study, the aqueous leaf extract of Justicia carnea was used. The percentage yield of the extract was 18.43. The proximate constituents of the extract were 4.61 ¬Ī ...

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PHYTOCHEMICAL CONSTITUENTS, FREE RADICAL SCAVENGING PROPERTIES AND ANTIDIABETIC POTENTIALS OF METHANOL EXTRACT OF THE STEM (BARK) OF ALSTONIA BOONEI ON ALLOXAN- INDUCED DIABETIC ALBINO RATS

ABSTRACT This  study  was  carried  out  to  investigate  the  phytochemical  constituents  of  the methanol extract of the stem (bark) of Alstonia boonei (MEAB), in vitro antioxidant activity of the extract and its possible antidiabetic  and antioxidant  potential using alloxan- induced diabetic rats as model. The qualitative analysis of the extract showed a  wide   range   of   phytochemicals,   which   could   be   physiologically   potent   in ameliorating several diseases. Quantitative  phytochemical  analysis revealed that ...

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SURVEY OF SODIUM POTASSIUM IRON AND CALCIUM CONTENTS OF RETAIL SAMPLES OF SOME PROCESSED FOODS (BREAKFAST CEREALS AND SNACKS) SOLD IN NSUKKA ENUGU STATE

ABSTRACT Processed  foods  have  been  noted  to  be  generally,  high  in  sodium  but,  low  in potassium. Some  of these foods such as snacks often provide a quick bite at work and school and often do not provide the needed nutrients such as calcium and iron needed in  adequate  quantities.   The  food  categories   sampled   were   biscuits,   sausages, cakes/pastries   and   breakfast   cereals.   Sodium,   potassium,    calcium   and   iron concentrations were determined within and ...

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EFFECT OF CHLOROFORM- METHANOL EXTRACT OF TETRACARPIDIUM CONOPHORUM NUTS (WALNUTS) ON OXIDATIVE STRESS MARKERS IN HYDROGEN PEROXIDE INDUCED WISTAR ALBINO RATS

ABSTRACT This work was done to ascertain the efficacy of the seed of Tetracarpidium conophorum on hydrogen peroxide induced oxidative stress in Wistar albino rats. Thirty five (35) male wistar albino rats weighing (120- 140g) were distributed into seven (7) groups of five  rats each. Groups 2-6 were administered with hydrogen peroxide (1.0 ml/kg), while group 1 served as the normal control while group 2 serves as positive control Groups ...

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PRODUCTION PARTIAL PURIFICATION AND CHARACTERIZATION OF CELLULASE BY ASPERGILLUS NIGER FROM SUBMERGED FERMENTATION OF GRAPE BAGASSE

ABSTRACT The increase in agricultural practices has necessitated the judicious use of agricultural wastes into value added products. In this study the ability of selected cellulosic substrate to induce cellulase production by Aspergillus niger and the ability of the induced enzyme to hydrolyze the cellulosic substrate were assessed. The enzyme was produced by submerged fermentation technique in which grape bagasse was the cellulosic substrate which served as a carbon source. ...

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THE EFFECT OF CONTINOUS FRYING ON DIFFERENT EDIBLE VEGETABLE OIL A COMPARATIVE STUDY

ABSTRACT Cooking was a breakthrough for mankind that improved the flavor, digestibility and quality of food. This breakthrough is seen in a lot of cooking methods such as boiling, poaching, stewing, baking, roasting, microwaving and deep frying. Heat treatment is an operation widely used in food processing. People especially in developing countries believe in the need to manage resources, while giving less attention to the risk associated with what is ...

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